LEARNING OUTCOMES
Aim and objective of the course: The use of proteolytic enzymes to treat unwanted hair growth. The study of proteolytic enzymes in relation to their penetration into the hair follicle, the study of how enzymes act on hair regenerative cells, the proteins that are found in small amounts and are necessary for the survival of cells.
Upon the successful completion of this educational module students are expected to be able to:
- know the method of enzymatic treatment of unwanted hair growth with proteolytic enzymes
- identify the type of hair growth, the amount of enzyme and how the enzyme will be used
- become familiar with the methods of treating unwanted hair growth depending on the aetiology that creates the increased hair growth
SYLLABUS
Theoretical Part of the Course
1. Anatomy and physiology of the skin. Generally about the hair.
2. Circular activity of the hair follicle. Hair regenerative cells and their location. Hair specificity by body areas.
3. Proteins – Enzymes. Characteristic properties of enzymes and their mode of action.
4. Classification of enzymes, allosteric modifiers. Enzyme analysis, how they work, enzyme kinetics.
5. Inhibition of enzyme reactions, pH effect, enzyme reaction rate.
6. Proteolytic enzymes with emphasis on trypsin, papain, chymotrypsin. Action of proteolytic enzymes with emphasis on the treatment of unwanted hair growth and ways of penetration.
7. Skin thresholds, how substances penetrate the skin. Iontophoresis as method, the device and ways and means of its use in the treatment of unwanted hair growth with enzymes.
8. Penetration of enzymes at different concentrations and for different iontophoresis enzymes.
9. Skin characteristics in the treatment of unwanted hair growth.
10. Pain and how to deal with it.
11. Conditions for permanent hair removal.
12. Experimental studies on the action of proteolytic enzymes on the skin of experimental animals.
13. Basic knowledge about transgenic mice, effect of proteolytic enzymes and results at the histological level of the skin.
Laboratory Part of the Course
1. Demonstration of the enzymatic method for the treatment of unwanted facial hair.
2. Learning the enzymatic method of treating unwanted hair growth in every skin type.
3. Enzymatic method of removing the unwanted hair growth, depending on the quality and the colour of the hair.
4. Enzymatic method of removing the unwanted hair growth in case that the increased hair growth is due to a hormonal problem
5. Application of enzymatic method after hair removal in hormone-dependent areas with methods of temporary treatment of unwanted hair growth and idiopathic aetiology.
6. Application of enzymatic method after hair removal in hormone-dependent areas with methods of temporary treatment of unwanted hair growth and hormonal aetiology.
7. Application of the enzymatic method in non-hormone dependent areas..
8. Application of the enzymatic method after the use of adhesive preparations.
9. Application of the enzymatic method without iontophoresis in hormone-dependent regions.
10. Application of the enzymatic method without iontophoresis in non-hormone-dependent regions.
11. Combinations of methods of permanent treatment of unwanted hair growth with the enzymatic one.
12. Application of the enzymatic method in combination with the use of Laser- IPL. Protocol implementation. Effectiveness.
13. Comparative study of the application effectiveness, based on laboratory recordings.