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LEARNING OUTCOMES
The student, with the successful completion of the course, should:
- know the types of instrumental methods of analysis.
- have acquired knowledge of the powerful and elaborate collection of tools, for qualitative and quantitative analysis of the matter.
- have understood the basic operation principles of measuring instruments.
- be able to compare the capabilities, the advantages and disadvantages of various analysis techniques.
- be able to understand the possible sources of errors, that follow the measurements.
- Understand the limitations of sensitivity, repeatability and accuracy of instrumental measurements.
SYLLABUS
THEORETICAL PART
Introduction
Classification of Analytical Methods-Types of Instrumental Methods of Analysis-Analytical Instruments- Selection of an Analytical Method- Calibration in Instrumental Methods- Signal and Noise
Atomic Spectroscopy I
Introduction to Spectroscopic Techniques- Organology- Atomic Absorption and Atomic Fluorescence Spectrometry- Atomic Emission Spectrometry
Atomic Spectroscopy II
Atomic Mass Spectrometry- X-ray Spectrometry
Molecular Spectroscopy I
Introduction to Ultraviolet / Visible Molecular Absorption Spectrometry (UV / Vis)- Applications of Ultraviolet / Visible Molecular Absorption Spectroscopy (UV / Vis)- Molecular Luminesence Spectrometry
Molecular Spectroscopy II
Introduction to Infrared (IR) Spectrometry- Applications of Infrared Spectrometry- Raman Spectroscopy
Molecular Spectroscopy III
Nuclear Magnetic Resonance Spectroscopy- Molecular Mass Spectrometry- Electron Microscopy
Separation Methods I
Introduction to Chromatographic Separations- Gas Chromatography
Separation Methods II
High Performance Liquid Chromatography (HPLC)
Separation Methods III
Extraction and Supercritical Fluid Chromatography- Electrophoresis and Capillary Electrochromatography
Electroanalytical Chemistry I
Introduction to Electroanalytical Chemistry- Coulometry
Electroanalytical Chemistry II
Voltametry- Potentiometry
Radiochemical and Thermal Methods
Automated Analysis Methods
LABORATORY PART
Ultraviolet / Visible Molecular Absorption Spectrometry: qualitative analysis, obtaining a spectrum of substance.
Ultraviolet / Visible Molecular Absorption Spectrometry: Quantitative analysis of proteins.
Atomic Emission Spectrometry: Quantitative analysis of Na+ and K+ quantification by flame photometry
Infrared (IR) Spectrometry: Analysis of uroliths.
High Performance Liquid Chromatography (HPLC), Reverse Phase: Quantitative determination of caffeine.
Automatic analyzer: Determination of enzymes, lipids, glucose and electrolytes.