LEARNING OUTCOMES
Students at the end of the lesson will be familiar with the:
- basic principles of enzyme immunoassays and chemiluminescence,
- way of calibration of immunoassays,
- use of immunoassay analyzers,
- hormones of pituitary gland, thyroid gland, adrenal glands, gonad glands,
- metabolism of calcium/phosphorus and the biochemical markers of osteoporosis,
- metabolism of iron and its biochemical markers,
SYLLABUS
Τheoretical courses
1. The kinetics of enzymes. The use of enzymes in diagnostic technology.
2. The metabolism of calcium, phosphorus and magnesium. The role of vitamin D.
3. The biochemical monitor of osteoporosis (markers of bone degradation and bone production).
4. The metabolism of iron. The importance of determining iron, iron-binding capacity, ferritin and transferrin.
5. Hormones of hypothalamus, pituitary and adrenal glands. The metabolic importance of hypothalamic hormones (GH-RH, Gn-RH, TRH, CRH) and anterior and posterior pituitary (TSH, LH, FSH, ACTH, PRL, GH, oxytocin, ADH).
6. The hormones of the thyroid gland. The metabolic significance and diagnostic role of T4, T3, TSH, TG, anti-TPO, anti-TG.
7. The hormones of the parathyroid gland and adrenal glands. The metabolic importance and the diagnostic role of the determination of parathyroid hormone (parathyroid gland) and cortisol, aldosterone, androgens, etc. (adrenal glands).
8. Hormones of male and female reproductive system. Hormones associated with sperm production and menstruation.
9. Biochemical monitor of pregnancy and prenatal control. The role of LH, FSH, estrogen, β-chorionic gonadotropin and other hormones that play a role in pregnancy.
10. The acid-base balance. The balance of water, sodium and potassium. Analytical methods for electrolytes’ measurement in laboratory.
11. The homeostasis of hydrogen ions. The balance of blood gases. The regulation of hydrogen ions. Analytical methods for blood gases’ measurement in laboratory.
12. Biochemical cancer markers. The role in the diagnosis and monitoring of anti-cancer treatment.
13. Drugs and their analytical measurement in laboratory.
Laboratory courses
1. Analytical measurement of serum iron with manual and automatic analytical method.
2. Analytical measurement of serum alkaline phosphatase by photometric kinetic method in photometers and biochemical analyzers.
3. Analytical measurement of lactic dehydrogenase in serum, CSF and other biological fluids. Training in immunochemistry analyzer.
4. Measurement of creatine kinase and CK-MB isoenzyme by photometric methods in photometers and biochemical analyzers. Training in immunochemistry analyzer.
5. Protein electrophoresis.
6. Enzyme electrophoresis.
7. The biochemical monitor of the peritoneal fluid. Determination of serum and peritoneal amylase. Training in immunochemistry analyzer.
8. The biochemical monitor of the cerebrospinal fluid (measurement of glucose, LDH and proteins).
9. Measurement of creatinine clearance.
10. Competitive immunoenzymatic methods. Training in a competitive ELISA.
11. Non-competitive immunoenzymatic methods. Training in a non-competitive ELISA.
12. Measurement of fructose in sperm samples. Training in an electrolytes’ analyzer.
13. Measurement of citric acid in sperm samples. The use of atomic absorption for the measurement of heavy metals.